Stemcell/EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit/RoboSep/18063RF
商品编号：
18063RF
品牌：
StemCells Inc
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
Advantages:
• Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour• No-wash removal of EasySep™ Releasable RapidSpheres™• Optional isolation of CD4+CD25- responder T cells from the same sample
Components:
- EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)
- EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
- EasySep™ Human CD127high Depletion Cocktail, 1 mL
- EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- EasySep™ Release Buffer, 2 x 1 mL
- RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)
- EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
- EasySep™ Human CD127high Depletion Cocktail, 1 mL
- EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- EasySep™ Release Buffer, 2 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
- EasySep™ EasyTube™-14 (Catalog #20128)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, CD4+; T Cells, Regulatory
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
品牌介绍
STEMCELL Technologies 成立于 1993 年，是加拿大最大的生物科技公司，为生命科学研究领域提供专业的细胞培养基和细胞分选产品。 我们的使命是为科学的不断进步提供高品质、创新的试剂、工具和服务，本着 Scientists Helping Scientists 的精神，用我们的服务帮助您的科学研究。 STEMCELL Technologies 为全球 70 多个国家提供超过 3000 种分子生物学工具，以支持干细胞、免疫学、癌症、类器官、基因编辑、细胞治疗和再生医学等领域的科学研究。我们的 mTeSR1™、MethoCult™ 和 EasySep™ 已经成为多能干细胞培养、造血干细胞鉴定以及无柱细胞分选的金标准；许多工具为广大的科研实验室所熟知。 此外，我们研发了一系列针对不同类器官的培养基，包括肠道类器官、脑类器官、肝类器官、胰类器官、上呼吸道类器官等，身体力行地推动类器官研究领域的发展。 STEMCELL Technologies，源于科研，归于科研。与您在科学的道路上携手前行！* 欲了解您区域的代理商信息，请通过电话 400-885-9050 或者邮箱 info.cn@stemcell.com 与我们联系。
